http://10.9.150.37:8080/dspace//handle/atmiyauni/367 2026-04-27T18:42:16Z 2026-04-27T18:42:16Z Rajpara, Roshniben Jayshukhbhai Dr. Anmol, Kumar http://10.9.150.37:8080/dspace//handle/atmiyauni/2329 2025-09-04T07:29:03Z 2025-07-01T00:00:00Z

Title: Studies on Isolation Characterization and Production of Fungal L Methionase a Promising Anti Cancer Agent From Soil Authors: Rajpara, Roshniben Jayshukhbhai; Dr. Anmol, Kumar Abstract: L-Methionase has emerged as a potent enzyme with promising applications in cancer therapy due to its ability to selectively deplete methionine an essential amino acid for methionine-dependent tumor cells. This study aimed to isolate and characterize fungal strains capable of producing Lmethionase, optimize its production, purify the enzyme, and evaluate its in vitro anticancer potential. Soil samples were collected from diverse ecological regions across Gujarat, India including marine, riverine, and agricultural sites to explore fungal biodiversity. A total of 50 fungal isolates were obtained, and qualitative screening using modified Czapek-Dox agar identified Aspergillus fumigatus MF13 as the most potent L-methionase producer. Quantitative assessment through enzyme assay and specific activity estimation further confirmed MF13’s enzymatic potential, with a maximum activity of 4.31 U/mL/min and a specific activity of 1.48 U/mg. Molecular identification using ITS sequencing validated MF13’s identity as Aspergillus fumigatus (GenBank accession: OQ690549). Optimization of enzyme production was achieved using a combination of One-Factor-at-a-Time (OFAT), Plackett-Burman Design (PBD), and Central Composite Design (CCD), culminating in a 2.57 U/mL/min yield under optimal conditions: 30°C, pH 8.0, 2.4 g/L yeast extract, and 1.2 g/L dipotassium phosphate. Purification via cold acetone precipitation and Sephadex G-75 chromatography resulted in a 10.5-fold increase in purity, with a specific activity of 40.0 U/mg and molecular weight of ~45 kDa, as confirmed by SDS-PAGE. Biochemical characterization showed optimal activity at pH 7.5 and 30°C, and notable stability under alkaline and moderate thermal conditions. Enzyme kinetics revealed a Km of 0.674 mM and Vmax of 0.871 U/mL, indicating strong substrate affinity. In vitro cytotoxicity assays (MTT) demonstrated dose-dependent anticancer activity of purified L-methionase. HT-29 (colon cancer) cells were highly sensitive (IC₅₀ ≈ 175 μg/mL), while MDA-MB-231 (breast cancer) cells showed resistance (IC₅₀ ≈ 390 μg/mL), suggesting variable methionine dependency. This research highlights Aspergillus fumigatus MF13 as a promising source of L-methionase and reinforces the enzyme's potential as a selective anticancer agent. The successful optimization and purification pave the way for further development in therapeutic applications, with future work focusing on overcoming resistance mechanisms and evaluating in vivo efficacy.

2025-07-01T00:00:00Z Vasant, Gunja Hiteshbhai Raghav, Ragini http://10.9.150.37:8080/dspace//handle/atmiyauni/2291 2025-01-25T09:51:00Z 2024-12-01T00:00:00Z

Title: Co-Application of Metal Oxide Nanoparticle(s) and Plant Growth Promoting Rhizobacteria on the Growth of Groundnut Plant (Arachis hypogaea L.) Authors: Vasant, Gunja Hiteshbhai; Raghav, Ragini Abstract: Groundnut (Arachis hypogaea L.) is an essential oilseed crop that plays a pivotal role in global agriculture and food security. However, its productivity is often restricted by minimal nutrient uptake, environmental stress, and dependency on chemical fertilizers. This study investigates an innovative, sustainable approach to enhance groundnut growth and productivity by integrating plant growth-promoting rhizobacteria (PGPR) and zinc oxide nanoparticles (ZnO NPs). The research aims to explain the synergistic effects of these biotic and abiotic agents on plant growth, and nutrient assimilation, laying a foundation for eco-friendly agricultural practices. Rhizospheres soil samples from groundnut fields in Saurashtra, Gujarat, were collected and analyzed. A total of 84 rhizobacterial isolates were screened for their ability to synthesize plant growth-promoting (PGP) compounds, including indole-3-acetic acid (IAA), ammonia, hydrogen cyanide (HCN), gibberellins, and phosphate-solubilizing activity. The most promising isolates were identified as Priestia megaterium (RGKP3), Bacillus haynesii (RG12), and Pseudomonas songnenensis (RG8) based on 16S rRNA sequencing and Gram staining. These strains demonstrated robust plant growth promoting (PGP) traits, contributing to root growth, nutrient availability, and stress mitigation. ZnO NPs were synthesized using the sol-gel method with zinc acetate as the precursor. Advanced analytical techniques, including UV-visible spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HR-TEM), were employed to characterize the crystalline structure, morphology, and optical properties of the nanoparticles. A concentration of 400 ppm ZnO NPs was determined as optimal for biological applications, ensuring compatibility with PGPR strains without inhibiting bacterial growth. The compatibility and synergistic effects of PGPR strains and ZnO NPs were assessed through growth curve analyses and seed priming experiments. Groundnut seeds treated with the combinations of PGPR and ZnO NPs were evaluated for germination rates, vigor index, and plant growth parameters under controlled conditions. Biochemical analyses were conducted to measure chlorophyll, carotenoid, flavonoids, sugars, proteins, and proline content, providing insights into photosynthesis, metabolic activity, and stress resilience. The combined application of PGPR and ZnO NPs significantly improved seed germination rates and vigor index, with Priestia megaterium (RGKP3) and ZnO NPs achieving the highest germination rate of 92%. Physical growth parameters, including root length, shoot length, number of leaves, and biomass, were noticeably enhanced by the combined treatments compared to individual applications. RGKP3+ZnO NPs resulted in the highest shoot and leaf development, while RG8+ZnO NPs were particularly effective in root proliferation. Biochemical analysis revealed significant improvements in chlorophyll, carotenoid, and flavonoid levels, reflecting enhanced photosynthetic efficiency and antioxidant activity. The combination of RGKP3 and ZnO NPs produced the highest chlorophyll and carotenoid concentrations, contributing to increased photosynthetic rates. RG8+ZnO NPs demonstrated remarkable increases in sugar and protein contents, indicating enhanced metabolic activity and stress mitigation. Reduced proline levels in treated plants underscored the role of these treatments in reducing stress. Among the three strains, Priestia megaterium (RGKP3) excelled in promoting photosynthetic efficiency and overall plant vigor. Pseudomonas songnenensis (RG8) exhibited a pronounced impact on biochemical traits, particularly in sugar and protein accumulation. Bacillus haynesii (RG12) contributed to plant growth and development but showed relatively moderate biochemical effects compared to RGKP3 and RG8. The integration of PGPR and ZnO NPs offers a sustainable approach to enhancing crop productivity while minimizing environmental impacts. By improving nutrient uptake, promoting growth, and mitigating stress, this strategy reduces the reliance on chemical fertilizers, aligning with the goals of sustainable agriculture. Priestia megaterium in combination with ZnO NPs demonstrated the highest potential for improving photosynthetic efficiency and plant vigor, whereas Pseudomonas songnenensis showed exceptional biochemical enhancements. These findings pave the way for broader agricultural applications, including field trials under diverse environmental conditions. Further research is recommended to optimize the formulations and explore the longterm effects of these treatments on soil health and crop yields. The study highlights the potential of integrating microbial inoculants with nanotechnology to revolutionize farming practices, ddressing global challenges in food security and environmental sustainability.

2024-12-01T00:00:00Z Patel, Shivani D. Bhattacharya, Chitra Pandhi, Neepa http://10.9.150.37:8080/dspace//handle/atmiyauni/2054 2024-11-26T06:57:46Z 2024-01-01T00:00:00Z

Title: Study of Effect of PGPR on Growth and Disease Resistance in Selected Economically Important Crops of Saurashtra Region- India Authors: Patel, Shivani D.; Bhattacharya, Chitra; Pandhi, Neepa Abstract: Usages of isolated plant growth promoting rhizobacteria as biofertilizer is an effective alternative for the sustainable agricultural practice to expand soil strength and to enhance the crop productivity of selected monocot and dicot plant seedlings. The aim of the present investigation is to explore a plant growth promoting bacteria with further employability of biofertilizer production applying on selected economically important crops (Cumin - Cuminum cyminum L., Rice - Oryza sativa, Mung - Vigna radiata L., Chickpea - Cicer arietinum L., and Groundnut-Arachis hypogaea) at a seedling stage. Total 75 bacterial isolates were collected from different region of Saurashtra, Gujarat state, India. Among them 41 morphologically different bacterial colonies were isolated and further screened for their PGPR traits. For the primary screening of selected bacterial isolates has been performed; IAA production, phosphate solubilization, siderophore production, HCN production and ammonia production. Among these 100% isolates were positive for ammonia production, 85.36% isolates were positive for IAA production, 31.70% isolates were positive for HCN production, 17.07% isolates were positive for phosphate solubilization, 9.75% isolates were positive for siderophore production at 37℃ of incubation period. In primary screening 11 bacterial isolates have showing PGPR properties but in quantitative screening of IAA & ammonia production, out of 11 only 4 bacterial isolates (KS2, KC8, KC9 and KC11) having the potency of PGPR traits. Bio-priming method was used for the seedling analysis. In current study to assess the impact of 4 specific PGPR isolates on seedlings of monocot (Rice, Mung, Cumin) & dicot (Groundnut, Chickpea) plants, we measured parameters like root and shoot length and no. of leaves have been calculated at the interval of 15 days under controlled conditions employing statistical analysis one factor at a time approach/CRD. Potent bacterial PGPR isolates were identified through 16S rRNA sequencing data analysis revealed two genera: Bacillus sp. and Proteus sp.Biological control agents are eco-friendly to prevent plant pathogens effectively. In the present investigation four potent isolates were examined for their ability to confer disease resistance against Fusarium sp induced wilt disease in monocot and dicot plant.In vitro technique was employed to inspect the ability of potent bacterial isolates such as KS2, KC8, KC9 and KC11 in order to control Fusarium sp. Result revealed by dual culture interaction shows, the colony diameter of Fusarium sp. was significantly reduced when KS2, KC8, KC9 interact with individually and KC11. However, the strain KC9- Bacillus sp. (OQ654027) had shown an antagonistic activity around 2.8mm found to be higher than other recorded strains in present study, while, KC11 Proteus Columbae sp. (OQ652027) had found to be recorded with an antagonistic activity around 2.3 mm against Fusarium sp. respectively. The other two strains namely, KS2 (Proteus Columbae) and KC8 (Bacillus sp.) had documented to show a decreased activity against the same strain as mentioned earlier. The two strains namely KS2 and KC8 although are found to be recorded with plant growth promoting activity but have not shown any significant antagonistic activity. The probable reason for the surprising result for KS2 and KC8 can be due to the fact of absence of ISR which found to be a strong reason for being an antagonistic agent.

2024-01-01T00:00:00Z Mashru, Riya Dr. Debashis, Banerjee http://10.9.150.37:8080/dspace//handle/atmiyauni/1430 2024-05-09T06:40:17Z 2023-12-01T00:00:00Z

Title: Study of Molecular Markers in Cervical Cancer and its Clinical Applications Authors: Mashru, Riya; Dr. Debashis, Banerjee

2023-12-01T00:00:00Z