http://10.9.150.37:8080/dspace//handle/atmiyauni/480 2026-04-27T18:59:16Z 2026-04-27T18:59:16Z Tank, Jigna G. Pandya, Rohan V. http://10.9.150.37:8080/dspace//handle/atmiyauni/2281 2025-01-04T06:55:58Z 2022-01-01T00:00:00Z

Title: Anti-proliferative activity of surfactins on human cancer cells and their potential use in therapeutics Authors: Tank, Jigna G.; Pandya, Rohan V. Abstract: Surfactins are cyclic lipopeptides that are isolated from various Bacillus strains. They are made up of heptapeptides and β-hydroxy fatty acids of variable chain lengths of carbon atoms. Therapeutically they are known to inhibit invasion, migration, and colony formation of human breast carcinoma cells. The role of surfactins is also known as anti-proliferative agents against human cancer cells through induction of apoptosis, arrest of the cell cycle, or suppression of survival signaling. The cytotoxic activity of surfactins is also perceived against human chronic myelogenous leukemia cells, human colon cancer cells, and hepatic carcinoma cells. Considering the wide spectrum of targets, the molecular effects of surfactins are diverse in different cancer cells and they can serve as promising chemotherapeutic agents for the treatment of cancer. Surfactins are being delivered to the targeted cancer cells through nano-carriers or nano-formulations. The present review article provides insight on different types and variations of surfactins, their molecular effect on different cancer cells, and their therapeutic use in the treatment of human cancer.

2022-01-01T00:00:00Z Singh, P. Patel, V. Shah, V. Madamwar, D. http://10.9.150.37:8080/dspace//handle/atmiyauni/2261 2025-01-02T07:06:16Z 2019-12-01T00:00:00Z

Title: A Solvent-tolerant Alkaline Lipase from Bacillus sp. DM9K3 and Its Potential Applications in Esterification and Polymer Degradation Authors: Singh, P.; Patel, V.; Shah, V.; Madamwar, D. Abstract: Solvent-tolerant lipase produced by Bacillus sp. DM9K3 has been isolated from the hypersaline area, White Rann of Kachchh, Gujarat, India. The strain initially showed lipase activity of 11.1 U/mL in a basal medium which increased to 52.0 U/mL under optimized culture conditions. Bacillus sp. DM9K3 exhibited stability at 7% salinity, pH 9.0 and 50°C. The extracellular lipase was partially purified by acetone precipitation followed by DEAE-cellulose resulting in 39-fold purification with 40% yield. Metals ions such as Mg2+, Ca2+ and K+ showed enhanced enzyme activity. EDTA did not have a significant effect on activity suggesting that lipase is not metalloenzyme. The lipase under study showed the highest activity when palmitate (C16) was used as a substrate and was also highly stable in organic solvents such as cyclooctane and benzene. The partially purified enzyme was immobilized for increasing the efficiency of the ethyl caprylate (an orange flavored ester) synthesis in the presence of cyclooctane. Additionally, lipase of Bacillus sp. DM9K3 was explored for biodegradation of polycaprolactone microspheres and showed promising results for potential applications in drug delivery system.

2019-12-01T00:00:00Z Singh, Prachi Jain, Kunal R. Shah, Varun Madamwar, Datta http://10.9.150.37:8080/dspace//handle/atmiyauni/2250 2025-01-02T06:02:33Z 2021-08-01T00:00:00Z

Title: White Rann of Kachchh harbours distinct microbial diversity reflecting its unique biogeography Authors: Singh, Prachi; Jain, Kunal R.; Shah, Varun; Madamwar, Datta Abstract: The understanding of sub-surface soil microbial diversity is limited at both saline and hypersaline ecosystems, even though salinity is found to affect the microbial community in aqueous and terrestrial environment. In this study, a phylo-taxonomy analysis as well as the functional characteristics of microbial community of flat salt basin of White Rann of Kachchh (WR), Gujarat, India was performed along the natural salinity gradient. The high throughput sequencing approach has revealed the numerical abundance of bacteria relative to the archaea. Salinity, TOC, EC and sulphate concentration might be the primary driver of the community distribution along the transect at WR. The much anticipated effect of salinity gradient on the microbial composition surprisingly turned out to be more speculative, with little variance in the community composition along the spatial distance of WR. The metabolic pathways involved in energy metabolism (like carbon, nitrogen, sulphur) along with environmental adaptive genes (like osmotic and oxidative stress response, heat and cold shock genes clusters) were abundantly annotated from shot-gun metagenomic study. The carbonic anhydrase harbouring bacteria Bacillus sp. DM4CA1 was isolated from WR, having a catalytic ability for converting the gaseous carbon dioxide in presence of calcium carbonate into calcite at 25 % higher rate as compared to non-harbouring strains. The enzyme has a role in multiple alternative pathways in microbial metabolism. With the array of results obtained, the study could become the new reference for understanding the diversity structure and functional characteristics of the microbial community of terrestrial saline environment.

2021-08-01T00:00:00Z Kurian, Noble K http://10.9.150.37:8080/dspace//handle/atmiyauni/2227 2025-01-01T12:38:51Z 2021-01-01T00:00:00Z

Title: Melanin and its Precursors as Effective Antiviral Compounds: Authors: Kurian, Noble K Abstract: Melanins are ubiquitous pigment which plays a diverse role in organisms ranging from bacteria to human beings. Its unique properties made it an important compound for study in diverse fields from electronics to drug discovery. Melanin and precursors have proved to be good antiviral candidate against many viruses. It has shown to inhibit replication of HIV-1 and HIV-2. Insilico studies proved that it can also interact with furin protein which is an important protein in SARS CoV2 pathogenesis. Our present study proved that melanin precursors like 5,6-dihydroxyindole-2-carboxylic acid (DHICA) and l-3,4-dihydroxyphenylalanine (L DOPA) strongly interacts with the spike protein of the CoV2. All these studies prove melanin and its precursors could be utilized as effective antiviral compounds

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