Exploration of possible mechanisms for anti-inflammatory activity of Ipomoea aquatica Forsk. (Convolvulaceae)

Abstract

Currently used steroidal and non steroidal anti-inflammatory drugs have severe side effects. These side effects are very difficult to manage than the disease itself. Hence, there is to search new safe resources to cure such diseases that the use of plant based drugs. This study deals with anti-inflammatory evaluation of the hydroalcoholic extract of Ipomoea aquatica leaves as well as their possible mechanism of action. A carrageenan‐induced rat paw oedema model was used for anti-inflammatory study. The mechanism/s by which Ipomoea aquatica is mediated the ant-inflammatory activity was determined by its effects in antihistamine activity, prostaglandin synthesis inhibition activity, membrane stabilizing activity and protein denaturation inhibition activity. Dose dependent anti-inflammatory activity was found with HAEIA in rat paw oedema model using carrageenan. HAEIA effective to suppressed the wheal area formed by histamine. HAEIA revealed dose dependent prostaglandin synthesis inhibition activity. HAEIA was effectively inhibited the heat induced hemolysis of HRBCs as well as heat induced albumin denaturation. Therefore, it was concluded that the HAEIA has anti-inflammatory activity possibly mediated through inhibition of release of mediator histamine and prostaglandin and has also HRBCs membrane stabilization and protein denaturation inhibition properties.