Cryopreservation of the outdoor algal culture

Abstract

Cryopreservation of algal culture refers to the process of freezing and storing algal cells at very low temperatures (-196°C) in liquid nitrogen for long-term preservation. The purpose of cryopreservation of algal culture is to maintain the genetic stability of the culture and to ensure that the culture can be revived in the future for further study or use. Cryopreservation provides a reliable and cost-effective method for long-term storage of algal cultures without the need for continuous sub-culturing, which can be time-consuming and labor-intensive. By preserving algal cultures in liquid nitrogen, using different cryoprotectant which prevent cell damage can ensure the availability of the culture for future research and development, even if the original culture is lost or contaminated. Additionally, cryopreservation allows for the preservation of a diverse range of algal strains, including rare and endangered species, which might otherwise be lost due to environmental changes, habitat destruction, or other factors. Easy revival of the cryopreserved culture under controlled parameters affecting algal growth. Revival culture further support to scale-up of culture which is pure strain.