In Vitro Diagnosis of rapid test

Abstract

Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices. Lateral flow immunochromatographic assays (LFIAs) are analytical devices used to detect the presence of one or more target analytes in a liquid sample. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients.the concept ofrapid diagnostic tests based on body fluids dated back significantly further.Documented evidence of saliva- and urine-based diagnostics existed severalthousand years ago. The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances.The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. The LFIA is a paper-based (bio)analytical technique for the on-site detection of target substances, where the sample is added on a standalone device and the result is obtained in a few minutes. LFA is performed over a strip, different parts of which are assembled on a plastic backing. These parts are sample application pad, conjugate pad, nitrocellulose membrane and adsorption pad. Lateral flow immunochromatographic assays (LFIAs) are analytical devices used to detect the presence of one or more target analytes in a liquid sample.