Isolation and screening of bacterial l-methionase from soil and dairy products

Abstract

Due to expression of oncogenes in normal cells, they get converted into tumor cells. Chemotherapy and radiotherapy used nowadays have many side effects and it kills normal cells. It has been established that a number of enzymes have anticancer property such as L-Asparaginase, L-Glutaminase, L-Arginase and L-Methionase. In this study, we focused on isolating and screening of bacterial L- Methionase enzyme from different soil samples and dairy products. During isolation from different soil samples and dairy products 14 isolates were purified and 6 of them showed positive results giving pink colored colonies. Out of 6 isolates, MGL 2, MGL 3, MGL 10 and MGL 13 showed maximum enzyme activities. MGL 3 showed highest enzyme activity of 8.38U/ml. In Morphological characterization MGL2, MGL 3, MGL 10 and MGL 13 showed purple color and are rod shaped Gram positive bacteria. In biochemical characterization four isolates MGL 2, MGL 3, MGL 10 and MGL 13 gave catalase test and methyl red test positive.