To Isolate and Screen River Bacteria to Check its Antagonistic Activity Against Antibiotic Resistant Pathogens

Abstract

River is one of the main biological resources and has potential to solve threat caused by antimicrobial resistance. From different river water sample and soil sample of Uttarakhand, India the microbes were isolated and screened to check it’s antimicrobial activities. The objective was to identified potential antibacterial from river sources which may help in treatment of antimicrobial resistance. The antimicrobial activity of isolates was checked against two pathogens: Salmonella typhi and Acinetobacter baumannii. Preliminary screening of river microorganism in opposition to antibiotic resistant pathogen is performed by cross streak technique. Secondary screening of positive isolates is done using Agar well diffusion technique, in addition a comparison was also made between centrifuged and non-centrifuged isolates to check whether the antimicrobial substance is produced externally or internally. A zone-of-inhibition was found in non-centrifuged plates around two isolates. Thus, further research on river bodies may lead to find better treatment of antimicrobial resistance.