Triterpenoid and Fatty Acid Contents from the Stem Bark of Cordia dichotoma (Forst f.)

Abstract

Aim: To isolate and determine the chemical constituents of the stem bark of Cor- dia dichotoma (Forst f.), a plant used for medicinal purpose in folk medicine. Materials and methods: Petroleum ether extract of the stem bark was used for this study. Saponification process was performed to separate fatty acid and unsa- ponifiable matter. Results: One triterpenoids, α-amyrin was isolated from the bark by using iso- cratic elution. The chemical compounds isolated, for the first time, were analyzed by GC/MS, IR, and UV. The chemical composition of the fatty acids methyl esters (FAMEs) in bark of Cordia dichotoma were also analyzed by gas chromatography- mass spectrometry. After methyl-esterification, 17 components were identified in the bark. The derivatization conditions were investigated in order to validate this method. Conclusion: The present analysis revealed that Cordia dichotoma stem bark con- tains 17 fatty acid. The principal themes of the review highlight the development and application of chromatographic techniques for the separation, isolation and detection of the compo