Invitro Study of Antagonistic Activity, Extraction and Optimization of Amylase Enzyme from Trichoderma Harzianum Against Selected Soil- Borne Fungal Isolates

Abstract

Trichoderma harzianum has been well known as a potent biocontrol agent against several plant pathogenic fungi. But information with regard to dual role of Trichoderma as biocontrol and plant growth promoting agent is less reported. The ϐ Ǥ ǡ present study was conducted to determine the myriad level of antagonistic activity against various soil borne fungal isolates. ϐ ǡ in Potato Dextrose Agar (PDA) supplemented with Streptomycin. Based on morphological and cultural characteristics, ʹ͵ ϐ ǡ ͳʹ Ƭ Ǥ ϐ ǡ Ǥ ǡ Ǥ ǡ Ǥ ǡ Ǥ ǡ Ǥ ǡ Ǥ ϔ ǡ Ǥ ǡ ǡ Ǥǡ Ǥǡ Ǥǡ and Fusarium oxysporum. Antagonistic screening activity had revealed that isolates of Trichoderma had shown the highest antagonism against 19 isolated fungal strains. It had also revealed that, Trichoderma harzianum had shown the minimum percentage of growth inhibition in a range of 11.11% to maximum of 94.11% among all selected soil borne fungal isolates. Trichoderma had also represented the highest antagonistic activity against sp. Further, extracellular enzyme viz. cellulase, amylase, pectinase, protease and chitinase activity had been determined qualitatively by zone of clearance, as representation of enzyme potency. Quantitative broth culture assay had revealed, maximum amylase activity 49.60U/ml. Further, optimum amylase activity was recorded