Development and Characterisation of Ayurvedic Polyherbal Formulation for Diabetic Wound Healing: A Comprehensive Study on Decoction, Phytochemical Analysis, and Topical Application

Abstract

Diabetic wounds represent a significant challenge in modern healthcare, with delayed healing and increased susceptibility to infections. This study aims to synthesise and characterise an Ayurvedic polyherbal formulation from Securinega leucopyrus (Katupila), Azadiracta indica (Limbdo), Acacia catechu (Khadir) and Vitex negundo (Nirgundi) for diabetic wound healing. The research encompasses preparation by decoction, followed by qualitative and quantitative phytochemical analysis, and biological assays including antioxidant, and antibiofilm activity against pathogens. Additionally, the study examines the development of a topical ointment for improved application on diabetes mellitus patients' wounds. The isolation and characterization of bioactive compounds are conducted using GCMS techniques