Please use this identifier to cite or link to this item: http://10.9.150.37:8080/dspace//handle/atmiyauni/883
Title: Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes micrococcus yunnanensis
Authors: Kumar, Ravi Ranjan
Jadeja, Vasantba
Keywords: Micrococcus yunnanensis strain rsk5
Catharanthus roseus
Endophytes
Antibacteria
Chromatography
Issue Date: 2017
Publisher: Journal of Pharmaceutical Analysis
Citation: Ranjan, R., & Jadeja, V. (2017). Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes Micrococcus yunnanensis. Journal of pharmaceutical analysis, 7(5), 343-347.
Abstract: Endophytic actinomycetes are considered as one of the relatively unexplored potential sources in search of antibiotic producer against antibiotic resistant pathogens. A potent strain isolated from Catharanthus roseus that displays antibacterial potential against antibiotic resistant human pathogen Staphylococcus aureus was characterized and designated as Micrococcus yunnanensis strain rsk5. Rsk5 is capable of producing optimum antibacterial metabolites on starch casein medium at 30 °C, pH 5 and 2% NaCl condition. The crude antibacterial agent was extracted from fermentation broth by ethyl acetate and separated by TLC using chloroform-methanol (24:1, v/v) solvent system with Rf value of 0.26. It was partially purified by flash chromatography, followed by HPLC and analyzed by ultraviolet visible spectrophotometer to get absorption maxima at 208.4 nm. The ESI-MS spectra showed molecular ion peaks at m/z 472.4 [M-H], which does not match with any known antibacterial compound.
Description: This work was supported by United Grant Commission, Pune, India (Grant no. 47-1328/10) for financial support. The authors are thankful to management of Shree M. & N. Virani Science College, Rajkot, India, for providing necessary research facilities.
URI: http://10.9.150.37:8080/dspace//handle/atmiyauni/883
ISSN: 2095-1779
Appears in Collections:01. Journal Articles

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